Time-resolved and Steady-state Fluorescence Spectroscopic Studies of the Human Lens with Comparison to Argpyrimidine, Pentosidine and 3-OH-kynurenine¶

Line Kessel; Stanislav Kalinin; Ram H. Nagaraj; Michael Larsen; Lennart B-Å Johansson

doi:10.1562/0031-8655(2002)076<0549:TRASSF>2.0.CO;2

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Line Kessel,^1,* Stanislav Kalinin,² Ram H. Nagaraj,³ Michael Larsen,¹ Lennart B-Å Johansson^2,*

¹aDepartment of Ophthalmology, Herlev Hospital, University of Copenhagen, Herlev, Denmark
²bDepartment of Chemistry—Biophysical Chemistry, Umeå University, Umeå, Sweden
³cCentre for Vision Research, Department of Ophthalmology, Case Western Reserve University Cleveland,

^**To whom correspondence should be addressed at (LK): Department of Ophthalmology, Herlev Hospital, University of Copenhagen, DK-2730 Herlev, Denmark. Fax: 45-44-53-86-69; line.kessel@dadlnet.dk*To whom correspondence should be addressed at (LBÅJ): Department of Chemistry—Biophysical Chemistry, Umeå University, S-901 87 Umeå, Sweden. Fax: +46-90-786 77 79; Lennart.Johansson@chem.umu.se

Abstract

The intrinsic fluorescence from the human lens on excitation in the UV region, referred to as blue lens autofluorescence, increases with age or in the presence of diabetes. The present study reveals that the relative contribution of compounds responsible for the blue autofluorescence appears to be a constant with age. Three potential candidates for the blue fluorescence were also studied with respect to fluorescence spectroscopic properties. These were argpyrimidine and pentosidine, both advanced glycation end products, and 3-hydroxykynurenine (3-OH-kynurenine), a photooxidative derivative of tryptophan. It was shown that the spectral properties of argpyrimidine and pentosidine are compatible with the observed blue fluorescence of the human lens, whereas the fluorescence from 3-OH-kynurenine is negligible.

Citation Download Citation

Line Kessel, Stanislav Kalinin, Ram H. Nagaraj, Michael Larsen, and Lennart B-Å Johansson "Time-resolved and Steady-state Fluorescence Spectroscopic Studies of the Human Lens with Comparison to Argpyrimidine, Pentosidine and 3-OH-kynurenine," Photochemistry and Photobiology 76(5), 549-554, (1 November 2002). https://doi.org/10.1562/0031-8655(2002)076<0549:TRASSF>2.0.CO;2

Received: 14 May 2002; Published: 1 November 2002

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